Effect of Terminal Electron Accepting Processes on Acetate Thresholds in Contaminated Sediments

Demonstrating the success of in situ bioremediation is challenging, requiring multiple lines of evidence, e.g., “footprints” of microbially-mediated processes that transform contaminants, like metabolic intermediates. This project evaluated the hypothesis that characteristic threshold concentrations of acetate, a key intermediate under anaerobic conditions, occur in contaminant plume regions dominated by different terminal…


CHAPTER 1 Introduction
1.1 In situ Bioremediation
1.2 Evaluating In situ Bioremediation
1.3 Increasing Importance for Evaluating Bioremediation
CHAPTER 2 Scope of Study and Objectives
CHAPTER 3 Literature Review
3.1 Demonstrating In situ bioremediation
3.2 Microbial Footprints
3.2.1 Concept
3.2.2 Key processes used to monitor footprints
3.2.3 Summary
3.3 Terminal Electron Acceptors
3.4 Redox potential
3.5 Use of acetate as an indicator of shifts in predominant TEAPs
CHAPTER 4 Materials and Methods
4.1 Experimental Materials
4.1.1 Sediment and Groundwater Collection
4.1.2 Sediment Slurry and Microcosm Preparation
4.2 Experimental Design and Methods
4.2.1 Characterization Experiment
4.2.2 Contaminated Sediment Acetate Threshold Determination
4.3 Analytical Methods
4.3.1 Oxidized Electron Acceptors and Reduced Products
4.3.2 Global TEAP Indicators
4.3.3 Aggregate Measurements
CHAPTER 5 Results and Discussion
5.1 Characterization Experiment
5.1.1 Nitrate Reduction
5.1.2 Dehalorespiration
5.1.3 Iron Reduction
5.1.4 Sulfate Reduction and Methanogenesis
5.1.5 Acetate Concentrations
5.1.6 Hydrogen Concentrations
5.2 Methanogenic Acetate Threshold Experiment
5.3 Dehalorespiration Acetate Threshold Experiment
CHAPTER 6 Summary and Conclusions

Author: Davis, Gayle

Source: University of Maryland

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