Genetics and Pathology Project Reports, Dissertation, Thesis, Working Papers, Pathology Research Papers, Resources and Information

Novel Methods for Synthesis of High Quality Oligonucleotides

The first part of the work describes a procedure of oligonucleotide purification using a reversed-phase cartridge. The developed method employs a very efficient yet mild oligonucleotide detritylation on the cartridge support allowing fast purification of oligonucleotides regardless of their 5ยด-modification. Thiol- and

Antibody-based Profiling of Expression Patterns using Cell and Tissue Microarrays

In this thesis, methods to study gene and protein expression in cells and tissues were developed and utilized in combination with protein-specific antibodies, with the overall objective to attain greater understanding of protein function.To analyze protein expression in in vitro cultured cell