Functional proteomics of protein phosphorylation in algal photosynthetic membranes

Oxygenic photosynthesis is a process of capture and conversion of sunlight into chemical energy by photoautotrophic organisms. Plants, green algae and cyanobacteria perform photosynthetic conversion of sunlight into chemical energy in the permanently changing natural environment. For successful survival and growth photosynthetic organisms have developed complex sensing and signaling acclimation mechanisms. The environmentally dependent protein phosphorylation in photosynthetic membranes is implied in the adaptive responses; however, the molecular mechanisms of this regulation are still largely unknown. We used a mass spectrometry-based approach to achieve a comprehensive mapping of the in vivo protein phosphorylation sites within photosynthetic membranes from the green alga Chlamydomonas reinhardtii subjected to distinct environmental conditions known to affect the photosynthetic machinery.The state transitions process regulating the energy distribution between two photosystems, involves the temporal functional coupling of phosphorylated light-harvesting complexes II (LHCII) to photosystem I (PSI). During state transitions several of the thylakoid proteins undergo redox-controlled phosphorylation-dephosphorylation cycles. This work provided evidences suggesting that redox-dependent phosphorylation-induced structural changes of the minor LHCII antenna protein CP29 determine the affinity of LHCII for either of the two photosystems…


1. Introduction
1.1. Photosynthetic energy conversion
1.2. Role of protein phosphorylation in adaptive responses of the photosynthetic apparatus
1.2.1. State transitions and phosphorylation of LHCII
1.2.2. PSII turnover and phosphorylation under high light stress
1.2.3. Chlamydomonas reinhardtii as a model organism for the adaptive response
studies of the photosynthetic apparatus
1.2.4. Adaptation of alga to nutrient limitation: carbon concentrating mechanis
2. Present investigation
2.1. Aim of the research
2.2. Metodological aspects
2.2.1. Mapping of protein phosphorylation
2.2.2. Vectorial proteomics approach
2.3. Exploring the phosphoproteome of photosynthetic membranes in Chlamydomonas reinhardtii
2.3.1. Specific protein phosphorylations induced at limited environmental CO2
2.3.2. Light-dependent protein phosphorylations
2.3.3. Multiply phosphorylated light-harvesting phosphoprotein CP29 is an integral part of the stress-response mechanism
3. Concluding remarks
4. Acknowledgements
5. References

Author: Turkina, Maria

Source: Linköping University

Download URL 2: Visit Now

Leave a Comment