surface plasmon resonance

A capture step with protein A is the most common purification step in the downstream purification process of monoclonal antibodies. It is therefore of great importance to increase the knowledge of the interactions involved in this purification technique. The purpose of this master thesis project was to determine the affinity of protein A domains to IgG subclasses by surface plasmon resonance (SPR). Besides the five homologous IgG-binding protein A domains (E, D, A, B, and C) an engineered domain, similar ...