Wet Organic Field Effect Transistor as DNA sensor

Label-free discovery of DNA has long been excellently exhibited on field effect transistor (FET) based devices. Because conducting organic materials was found and have lured a lot more research efforts by their deep benefits, this report will concentrate on using an organic field effect transistor (OFET) as DNA sensor. An OFET designed with a transporting fluidic channel, WetOFET, forms a fluid-polymer (active layer) interface where the probe DNA could be introduced. DNA hybridization and non-hybridization after injecting target DNA and non-target DNA had been closely watched by transistor characteristics. The Hysteresis area of transfer curve improved after DNA hybridization that may be due to the growing electrostatic screening induced by the increasing negative charge from target DNA. The different morphology of coating surface may possibly also impact the OFET response…

Contents: Wet Organic Field Effect Transistor as DNA sensor

1 Introduction
1.1 Background and Goal
1.1.1 Background
1.1.2 Aim of This Work
1.1.3 Outline
1.2 Theoretical Background
1.2.1 Conjugated Polymers
1.2.2 Organic Field-Effect Transistor
1.2.3 Fluorescence
1.2.4 DNA and Hybridization
2 Architecture of Wet Organic Field-Effect Transistor
3 Experimental Details
3.1 Material
3.1.1 Poly(3-hexylthiophene)
3.1.2 Cholesterol Tag
3.1.3 DNA samples
3.1.4 PDMS
3.1.5 Phosphate Buffered Saline (PBS)
3.1.6 Experimental setup
3.2 Spin Coating
3.3 Measurements Results and Discussion
4.1 General results
4.1.1 Transfer characteristics
4.1.2 Output characteristics
4.1.3 Water and PBS
4.2 Fluorescence Measurement
4.3 DNA Detection
4.3.1 Overview
4.3.2 Non-complementary DNA versus Complementary DNA

Source: Linköping University

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