The assembly of ribosomal RNA (rRNA) and ribosomal proteins (r-proteins) into ribosomal subunits (30S and 50S) is actually a complex process. Transcription of rRNA needs antitermination proteins and the main transcripts are processed by ribonucleases. R-proteins and rRNAs are chemically altered, the r-proteins bind to the rRNAs and the formed RNA-protein complexes are folded into mature ribosomal subunits. These processes are well-coordinated and overlapping. Non-ribosomal factors are needed for proper assembly and maturation of the ribosomal subunits. A couple of these factors are the RimM and RbfA proteins that bind to 30S subunits and therefore are essential for successful processing of 16S rRNA. Lack of either RimM or RbfA results in a reduced amount of polysomes and a lower growth rate…
Contents
2 ABSTRACT
3 PAPERS IN THIS THESIS
4 INTRODUCTION
4.1 STRUCTURE AND FUNCTION OF RIBOSOMES.
4.2 IN VITRO ASSEMBLY OF RIBOSOMES.
4.3 REGULATION OF RIBOSOMAL SYNTHESIS.
4.4 PRODUCTION OF RIBOSOMES IN VIVO.
4.5 PROCESSING OF RIBOSOMAL RNA.
4.6 MODIFICATIONS OF RIBOSOMAL COMPONENTS.
4.7 ACCESSORY FACTORS FOR RIBOSOMAL ASSEMBLY.
5 AIMS OF THIS THESIS
6 RESULTS AND DISCUSSION
6.1 RLMB IS REQUIRED FOR RRNA METHYLATION BUT NOT FOR RIBOSOMAL ASSEMBLY.
6.2 MUTAGENESIS OF RIMM AND ISOLATION OF SUPPRESSOR MUTATIONS.
6.3 INTERACTIONS BETWEEN RIMM AND RIBOSOMAL COMPONENTS.
6.4 RBFA AND SUPPRESSION OF ∆RIMM.
6.5 STRUCTURAL AND FUNCTIONAL STUDIES OF RBFA….
Source: Umea University
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