In this thesis interactions in complex fluids, such as serum and meat juice, were analysed with optical biosensor techniques.Panels of lectins immobilised on gold surfaces were used for investigation of differences in protein glycosylation pattern in sera and meat juices between various species. The present panel was also used for investigation of global glycosylation changes of serum proteins in type 1 diabetes patients. Biorecognition was evaluated with null ellipsometry and scanning ellipsometry combined with multivariate data analysis techniques (MVDA). Principal component analysis (PCA) showed that the lectin panel enabled discrimination between sera from the different species as well as for the different meat juices….
Contents
1. Introduction
2. Biomolecules and biomolecular interactions
2.1 Proteins
2.1.1 Antibodies
2.1.2 Lectins
2.2 Serum
3. The immune system in short
3.1 Autoimmunity and autoantibodies
4. Diabetes
4.1 Type 1 diabetes
4.2 IAA and insulin
5. Sensor surfaces: protein immobilisation and non-specific binding
5.1 Immobilisation methods
5.1.1 Adsorption
5.1.2 Controlled protein immobilisation
5.2 Strategies to combat non-specific binding
5.2.1 Dilution
5.2.2 Pre-separation
5.2.3 Surface chemistry
5.2.4 Additives
5.2.5 Blocking
5.2.6 Heat treatment
6. Optical biosensor techniques
6.1 Ellipsometry
6.1.1 Scanning ellipsometry
6.2 Surface plasmon resonance
7. Immunoassays
7.1 Direct binding
7.2 Sandwich assay
7.3 Displacement assay
7.4 Indirect competitive inhibition
7.4.1 An indirect competitive immunoassay for IAA based on SPR
7.5 Radioimmunoassay (RIA)
8. Data analysis
8.1 Principal component analysis
8.2 Artificial neuronal networks
8.3 Bioanalytical methods and MVDA
9. Included papers
10. References
Author: Carlsson, Jenny
Source: Linköping University
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