The goal of this research is to elucidate the mechanism of virus recognition in molecularly imprinted polymers (MIPs) using already utilized techniques. The clinical relevance of this study relates to the development of a virus imprinted MIP, which would apply to the identification, classification, and removal of viruses…
Contents
1 Introduction
1.1 Significance
1.2 Background
1.3 Mechanism of molecular imprinting
1.4 Model viruses used in this work
1.5 Summary
2 Synthesis of virus imprinted polymers for the recognition of Tobacco Mosaic Virus
2.1 Introduction
2.2 Materials and Methods
2.2.1 Materials
2.2.2 Virus Purification
2.2.3 Hydrogel Synthesis and Template Extraction
2.2.4 Template Extraction
2.2.5 Virus Binding Test
2.3 Results and Discussion
2.4 Conclusions
3 Optimization of virus imprinting technique to improve selectivityand reduce non-specific binding
3.1 Introduction
3.2 Materials and Methods
3.2.1 Materials
3.2.2 Virus purification and fluorescent labeling
3.2.3 Virus Aggregation Studies
3.2.4 Fluorescent Virus MIP Synthesis
3.2.5 Template Removal Study from Virus MIP
3.2.6 Wildtype TMV MIP synthesis
3.2.7 PAA/EGDE hydrogel swelling studies
3.2.8 Virus binding test
3.3 Results
3.3.1 Aggregate formation in the polymer-virus solution
3.3.2 Virus removal studies
3.3.3 PAA/EGDE crosslinked hydrogel swelling studies
3.3.4 Virus binding studies
3.4 Conclusions
4 Future work
Author: Bolisay, Linden De Venecia
Source: University of Maryland
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