Thymine dimers are premutagenic lesions that form via ultraviolet irradiation of DNA. While the distribution of thymine dimers is non-random, it is also not yet predictable. Thymine dimer accumulation is likely to be controlled by both its formation and reversion. Formation of thymine dimers occurs via direct excitation of thymine residues, while the reversion is governed by…
Contents
CHAPTER 1
BACKGROUND AND SIGNIFICANCE
1.1 Introduction to Photodamage caused by UV light
1.2 The Chemistry and Biochemistry of Cyclobutane Pyrimidine Dimers
Types of Dimers
Thymine Dimer Repair
Variablility of T^T Formation
1.3 Charge Transfer in Biological Systems
Hole Transfer
Excess Electron Transfer
1.4 Specific Aims
CHAPTER 2
REVERSIBILITY OF T^T IN A BIOLOGICALLY RELEVANT SYSTEM
2.1 Higher Order DNA Structures
Nucleosome Structure
2.2 Experimental
Materials
General Methods
2.3 Results and Discussion
5s DNA
Photochemistry of Reconstituted 5s DNA.
Widom 601 Nucleosome Positioning Sequence
CHAPTER 3
FUNDAMENTALS OF EXCESS ELECTRON TRANSPORT
3.1 Charge Transfer (CT) Mechanisms
Hole Transport (HT)
Excess Electron Transport Mechanism
EET Donors
3.2 Experimental
Materials
Methods
3.3 Results and Discussion
Delocalization of EET by T-tracts: Polaron-Type Mechanism
3.4 Summary and Conclusion
CHAPTER 4
SUMMARY AND FINAL DISCUSSION
APPENDIX I
SUPPORTING INFORMATION FOR CHAPTER 2
APPENDIX II
SUPPORTING INFORMATION FOR CHAPTER 3
BIBLIOGRAPHY
Author: Finch, Amethist Shajary
Source: University of Maryland
Download URL 2: Visit Now